Fig. 6: Derepression of β cell-disallowed genes and their mRNA stabilization in Cnot3βKO islets. | Communications Biology

Fig. 6: Derepression of β cell-disallowed genes and their mRNA stabilization in Cnot3βKO islets.

From: Loss of β-cell identity and diabetic phenotype in mice caused by disruption of CNOT3-dependent mRNA deadenylation

Fig. 6

a GSEA of β-cell-disallowed genes, identified in Pullen et al.19, in Cnot3βKO islets. b Heatmap of 30 disallowed genes showing mRNA expression obtained from RNA-seq analysis of control and Cnot3βKO islets. c Decay curves of the indicated mRNAs. Total RNA was prepared from control and Cnot3βKO islets treated with Act D for 0, 4, or 8 h. Relative mRNA levels were determined by qPCR and normalized to the Gapdh mRNA level. mRNA levels without Act D treatment (0 h) were set to 100% (n = 3). d RIP-qPCR in MIN6 cells using normal anti-mouse and anti-CNOT3 antibodies revealing the interaction of CNOT3 with the indicated mRNAs that adversely affect β cell function (n = 3). Data are presented as mean ± SEM; *P < 0.05; **P < 0.01; ***P < 0.001, two-tailed Student’s t test.

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