Fig. 5: Expression, localisation and interactions of the Trichuris suis BCL-2-like proteins A and B.
From: Diversity in the intrinsic apoptosis pathway of nematodes
a HA-tagged T. suis A expressed at similar levels to mammalian BAX and BAK when introduced into Bax−/−/Bak−/− MEF, while expression levels of T. suis B was lower. b Both T. suis BCL-2-like proteins localised to the mitochondria-containing pellet fraction (P) similar to mammalian BAK and BCL-2, whereas BAX is localised in both cytosolic soluble (S) and pellet fractions. c HA- and FLAG-tagged T. suis A and B did not interact under any of the detergent lysis conditions tested unlike BAK and MCL-1 which co-immunoprecipitated in the presence of Triton X-100. WB: western blot, WCL: whole-cell lysate, IP: immunoprecipitation. Uncropped blots used to construct figures are provided in Supplementary Figs. 1–3.
