Fig. 2: E. coli markedly increase in CFDfl/flpvillin-creT mice.
From: Reg4 and complement factor D prevent the overgrowth of E. coli in the mouse gut
a Map of complement-mediated bactericidal activity. b The proportion of gut bacteria after 16s rRNA analyses of colon contents in normal CFDfl/flpvillin-crew (WT) and CFDfl/flpvillin-creT (CFDKO) mice (pooled samples from six mice). Also see Fig. S3A. c The proportion of gut bacteria after 16s rRNA analyses in CFDfl/flpvillin-crew (WT) and CFDfl/flpvillin-creT (CFDKO) mice after 2% DSS. Also see Fig. S3B. d The proportion of E. coli (Escherichia-Shigella) in CFDfl/flpvillin-crew (WT) and CFDfl/flpvillin-creT (CFDKO) mice after 2% DSS (n = 5). Also see Fig. S3C. e Flow cytometry of feces bacteria after staining using anti-LPS antibodies in CFDfl/flpvillin-crew (WT) and CFDfl/flpvillin-creT (CDKO) mice after 2% DSS. f QPCR of colonic content (upper) and colonic tissues (lower) in CFDfl/flpvillin-crew (WT) and CFDfl/flpvillin-creT (CFDKO) mice after 2% DSS (n = 6). g FISH of E. coli in the gut tissues of CFDfl/flpvillin-crew (WT) and CFDfl/flpvillin-creT (CFDKO) mice after 2% DSS. One representative from six mice. Scale bar, 40 μM; Scale bar in bottom panel, 5 μM. Two side student’s t test in e and f; *P < 0.05; **P < 0.01; ***P < 0.001; NS no significance.
