Fig. 2: Long-term oral AA supplementation prevent the evolution of myeloid neoplasia (MN) in Tet2+/− mice.
From: Context dependent effects of ascorbic acid treatment in TET2 mutant myeloid neoplasia
a Both WT and Tet2+/− mice were treated with 4.5 Gy of ionizing radiation. WT mice were maintained on H2O. Tet2+/− mice were supplied or not supplied with 3.3 g/L AA in H2O. b Kaplan–Mayer survival curves for WT and Tet2+/− mice. AA treatment significantly prolonged survival in irradiated Tet2+/− mice. c Long-term AA treatment of irradiated Tet2+/− mice reduced liver myeloid cell infiltration (top) and Gr1+/Mac1+ BM cell proportions (bottom). The figures are representative results of 8 mice for each group. d Tet2+/− and Gulo−/− mice were crossed to create hybrid Tet2+/−;Gulo−/− mice. These mice were given either 0.033 g/L or 3.3 g/L ascorbic acid in their drinking water for six weeks, then sacrificed. e–g A 3.3 g/L AA increased DNA oxidation levels in Tet2+/−;Gulo−/− mice. DNA oxidation products (E. 5hmC, F. 5fC, and G. 5caC) were assessed by 2D-UPLC-MS/MS. Data are shown as mean with SEM (n = 6). h Tet2+/−;Gulo−/− mice were maintained on either 0.033 g/L AA, 0.33 g/L AA, or 3.3 g/L AA in their drinking water for 1 year. i 3.3 g/L AA prolonged survival of Tet2+/−;Gulo−/− mice compared to 0.033 or 0.33 g/L AA. Curve comparisons were performed in GraphPad Prizm 8.0.1 and the p values are shown for Gehan-Breslow-Wilcoxon test (b and i) and statistical significance (p values) from two tailed t test are indicated (e–g).
