Fig. 5: Size exclusion chromatography elution profile of PaqFOS1 monitored at 280, 254, and 450 nm corresponding to absorbance (mAU) of the polypeptide, NADPH, and FAD, respectively. | Communications Biology

Fig. 5: Size exclusion chromatography elution profile of PaqFOS1 monitored at 280, 254, and 450 nm corresponding to absorbance (mAU) of the polypeptide, NADPH, and FAD, respectively.

From: A flavin-dependent monooxygenase catalyzes the initial step in cyanogenic glycoside synthesis in ferns

Fig. 5

Based on the elution volume in comparison to a set of reference proteins with known molecular masses, the PaqFOS1 protein eluted as a dimer with a mass of ~120 kDa. SDS-PAGE analysis of the PaqFOS1 containing fractions obtained by size exclusion chromatography demonstrating that PaqFOS1 migrated with an apparent molecular mass of 60 kDa in agreement with a calculated molecular mass of 62.5 kDa for the monomeric protein.

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