Fig. 4: Cellular decrease of expressed ORs with higher differentiation status.

a Monocle-generated pseudotemporal trajectory of malignant breast epithelial cells of luminal A molecular subtype of breast carcinoma, depicting the decrease in cellular stemness during cellular differentiation time-course (pseudotime). The cells with high stemness properties were selected as a starting point. Arrowheads indicate the direction of cellular differentiation across the pseudotime. Scale bar represents the pseudotime. b Uniform Manifold Approximation and Projection (UMAP) represents the decrease in the number of expressed OR genes per malignant cell in luminal A subtype in breast carcinoma during pseudotemporal trajectory. Scale bar represents the number of expressed OR genes per cell. Arrowheads indicate the direction of cellular differentiation across the pseudotime. c Scatter plots depicting an overall decrease in the expression of representative ORs along the pseudotemporal trajectory in the indicated conditions. Scale bar represents the number of expressed OR genes per cell. d Pseudotemporal trajectory of healthy luminal breast epithelial cells. Arrowheads indicate the direction of cellular differentiation. Scale bar represents the pseudotime. e Uniform Manifold Approximation and Projection (UMAP) representing the number of expressed OR genes per cell in the healthy luminal breast epithelial cells along the pseudotemporal trajectory. f Scatter plots depicting the expression dynamics of representative ORs along the pseudotemporal trajectory in the indicated conditions.