Fig. 5: Colonization of the omentum is disrupted by CCR1 knock-down in the cancer cells. | Communications Biology

Fig. 5: Colonization of the omentum is disrupted by CCR1 knock-down in the cancer cells.

From: Omental macrophages secrete chemokine ligands that promote ovarian cancer colonization of the omentum via CCR1

Fig. 5

a Schematic of experimental design for the 24hpi and 7dpi of ID8 in vivo metastasis assay. b Representative sections of omenta collected at 24 hpi and 7 dpi of stained for CK8/18 by IHC (scale bar = 100 µm). c Quantification of DAB staining area as an indicator of ID8 cancer cell burden in omental tissues. d Quantitation of flow cytometry analysis of ID8-mRUBY cells colonizing omentum at 24 hpi and 7 dpi (*p < 0.05). e Kaplan–Meier curve showing percent-survival after i.p injection of ID8- parental, CRISPR-Control, or CCR1-null cells (**p < 0.001) (n = 6/group). f Representative H&E stained peritoneal tissues at experimental end point. H&E images of ID8-CCR1-null group are from mice euthanized at 97 days, the experimental end point of the ID8-CRISPR-Control group. (scale bar = 100 µm). g Examples of 3 patient cores of endometrioid histology from a tissue microarray stained for CCR1 and IHC quantitation of CCR1 levels in a tissue microarray with different ovarian cancer histologies (****p < 0.0001). h, i Kaplan–Meier survival analysis (log-rank test) of CCR1 expression on progression free survival of serous ovarian cancer patients in h all stages and i stages I and II.

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