Fig. 1: Comparison of the conjugation rates of 13 plasmids under different conditions.

A set of 13 conjugative plasmids were tested for their ability to transfer between two EcN cells during 2 h on agar (n = 3, biologically independent samples) (a), in broth (n = 3, biologically independent samples) (b), and for 24 h in situ (n = 4, biologically independent mice) (c) in a murine gut conjugation model. The dotted vertical line highlights a conjugation rate that allows all recipient cells to receive a plasmid. Asterisks denote the absence of detectable transconjugant colonies. White circles show individual values for each dataset. Bar graphs represent the average and error bars the standard deviation of the data set.