Fig. 6: Effect of dsRNA treatment on DENV3 loads and Wolbachia density.
From: Dengue virus dominates lipid metabolism modulations in Wolbachia-coinfected Aedes aegypti
To validate the functional role of certain lipid classes on DENV3 replication, WT and wMel mosquitoes were injected with DENV3 in parallel with either anti-GOI dsRNA (orange circles) or control dsRNA (maroon squares). a–d Viral loads were quantified by qRT-PCR as genome copies per μg of extracted RNA at 4 days post-injection (n = 5 mosquitoes per condition). To validate the functional role of certain lipid classes on Wolbachia replication, wMel mosquitoes were treated with either control dsRNA (green circles) or with anti-GOI dsRNA (blue squares), with and without DENV3. e–h Relative Wolbachia densities were quantified by qRT-PCR as the copy number ratio of the Wolbachia gene TM513 to the mosquito gene RpS17 in genomic DNA at 4 days post-injection (CRLS: n = 5 mosquitoes per condition; PLD3, PNLIP, VLDLR: n = 10 mosquitoes per condition). Mean and standard error are shown in graphs. Each data point represents one mosquito. P values are shown where post hoc comparisons were made (Student’s two-sided t test with Bonferroni correction).
