Fig. 4: Co-expressed FtsA and ZapA organize FtsZ into long membrane-tethered bundles within liposomes.
From: Cell-free biogenesis of bacterial division proto-rings that can constrict liposomes
a Schematic illustration of liposome reconstitution assays with 3 µm purified FtsZ-A647 and co-expression of the ftsAopt and zapAopt DNA constructs. b Confocal fluorescence images of liposomes exhibiting membrane recruitment of FtsZ-A647 after 3 h incubation. Fluorescence from the membrane dye is colored in green and FtsZ-A647 signal is in magenta. Only the composite images are displayed. Arrows indicate membrane septa with co-localized FtsZ. Scale bars represent 5 µm.
