Fig. 1: Gene chip analysis of laser capture-microdissected SCNs in PACAP−/− and wild-type mice illuminated or not illuminated with light in the late subjective night (CT 21). | Communications Biology

Fig. 1: Gene chip analysis of laser capture-microdissected SCNs in PACAP−/− and wild-type mice illuminated or not illuminated with light in the late subjective night (CT 21).

From: Lipocalin-type prostaglandin D synthase regulates light-induced phase advance of the central circadian rhythm in mice

Fig. 1

a mRNA expression levels of the 539 differentially expressed genes as measured by hybridization signal intensity. Clustering dendrograms show the relative expression values according to the scale shown on the bottom left side (magenta, high expression level; light green, low expression level). b Venn diagram illustrating pairwise overlap of the genes. Data in each genotype comparison with light stimulation represent upregulated (>1.7-fold change) or downregulated (>0.6-fold change) genes. The 18 genes annotated to the term “extracellular region” are indicated by a purple dotted circle. These genes were upregulated by light stimulation in wild-type mice and are listed in Table 1. Real-time quantitative PCR analysis for L-Pgds and Cryab in the SCN upon light stimulation at CT 21 (c) and CT 15 (d). The Per1 and Prok2 genes were used as positive controls. ND indicates not detected. The values are shown as the mean ± SEM (n = 4–7 per group). Statistically significant differences were assessed using two-way ANOVA followed by Tukey–Kramer tests. *p < 0.05; **p < 0.01, vs kept without light stimulation.

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