Fig. 3: Overview of the animal experiments and the employed bactoline assay. | Communications Biology

Fig. 3: Overview of the animal experiments and the employed bactoline assay.

From: Deep phenotypic characterization of immunization-induced antibacterial IgG repertoires in mice using a single-antibody bioassay

Fig. 3

a Mice were immunized with 108 HK-B in alum (or alum alone); and after 6 weeks boosted with the same immunization. Six days afterward, spleens were extracted and cells from the B-cell lineage were purified. Cells were split, and half of them were measured using a beadline assay (for measurement of secretion rates and frequencies of IgG- and IgM-SCs) and the other half in bactoline assays to measure bacteria-binding IgG. b Example curves of individual cells from a bactoline assay. Shown are fluorescence relocation values extracted from five different individual cells that secreted an IgG binding to HK-B. The micrograph shows the bright field and corresponding fluorescence image of a IgG-SC (full circle data) at time 0 and 60 min.

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