Fig. 5: Cross-reactivity of commercial and immunization-induced IgGs.

a Cross-reactivity of the used commercial antibodies (Fig. 2); *p-value < 0.05; **p-value < 0.01; ***p-value < 0.001. All data in a is shown as Tukey box-and-whisker plots; the corresponding marker depicts the mean relocation (n > 3000 droplets for each point). b Frequency of cross-reactive antibodies in immunized mice. Data are shown as the mean frequency ± standard deviation over all mice (n = 3 per condition). c Characterization of cross-reactive IgG in HK-ST immunized, and d HK-EB immunized mice. Data are shown as in Fig. 4d (for each panel 200 > n_{anti-HK-B IgG-SCs} > 2). Frequencies of cross-reactive IgGs are shown as a measure of relative frequencies of anti-immunogen IgG-SCs (red, b).