Fig. 4: Cdh6/8 DKO phenotype at the Fb/Mb compartment boundary.
From: Redundant type II cadherins define neuroepithelial cell states for cytoarchitectonic robustness
a–e Cranial regions from E10.5 embryos are stained by anti-Pax6. Scale bar: 100 μm. An enlarged image around the wild type (WT) Fb/Mb boundary indicated by the frame b is shown in b. Note that Pax6 expression limit sharply delineates the Fb/Mb boundary. c, d Sharp boundaries are still maintained in Cdh6 KO; Cdh8+/− and Cdh6+/−; Cdh8 KO mice. e In Cdh6/8 DKO mice, Pax6 expression limits turn to be fuzzy and intermingled. Arrowheads indicate clusters of Pax6-positive cells that invade the Mb. f Bar graph shows the average total length of Pax6 expression boundaries (Length of the Fb/Mb boundary = 1.0) in each genotype specified below. Biologically independent sample numbers (n) are also indicated at the bottom. All data points are shown along with the mean ± s.d. **P < 0.01. g Results from b–e are summarized. At the upper most portion of this panel, Cdh expression levels along the A-P axis are indicated by the depth of characters or double-headed arrows. Distribution patterns of Pax6-positive (+) and negative (–) cells at around the Fb/Mb boundary are shown by brown and white circles, respectively. Note that the Fb/Mb boundary is disrupted only when mutually exclusive Cdh expression is eliminated at both sides.
