Fig. 2: Identification of protein antigens for tumor-specific dominant B cell clones. | Communications Biology

Fig. 2: Identification of protein antigens for tumor-specific dominant B cell clones.

From: Focal adhesion ribonucleoprotein complex proteins are major humoral cancer antigens and targets in autoimmune diseases

Fig. 2

a A schematic summary of our screening strategy. Following the discovery of tumor-specific and dominant chronotypes of tumor-infiltrating B cells by repertoire sequencing, their protein antigens were biochemically explored. b–g All the protein antigens identified in this study are shown. The upper panels show Coomassie Brilliant Blue (CBB)-stained SDS-PAGE results of immunoprecipitation (IP) using reconstructed human immunoglobulins. The lower panels show confirmative immunoblots of immunoprecipitated lysates of independent IP experiments using commercial antibodies. HGC27, GSU, NUGC3, MKN1, and KATOIII are human GC cell lines; HL60 and THP-1 are human promyelocytic leukemia cell and leukemic monocyte, respectively; and L23immo is an immortalized human fibroblast cell line. “Ctrl” represents the immunoglobulin-only lane. IP experiments were performed at least twice.

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