Fig. 2: Intrinsic characteristics of MB cell lines with respect to lymphangiogenesis marker expression.
From: VEGFC negatively regulates the growth and aggressiveness of medulloblastoma cells
a Average Daoy and HD-MB03 cell proliferation. Daoy or HD-MB03 cell proliferation was measured every day for eight days. n = 3 experiments; **p < 0.01 (Mann–Whitney analysis with Holm-Sidak correction). b Representative experiment of 24 h-directional migration of Daoy or HD-MB03 cells in Boyden chambers. Migrated cells were colored with Giemsa. Scale bar = 500 µm. c Basal relative amount of VEGFC mRNA and lymphangiogenic related genes in Daoy and HD-MB03 cells, as measured by RT-qPCR. n = 4 experiments; ***p < 0.001 (ANOVA, with multiple comparisons). d Basal amount of VEGFC secreted by Daoy or HD-MB03 cells. n = 6 experiments; p < 0.001. e Representative experiments showing the expression of VEGFC in both Daoy and HD-MB03 cells. VEGFC levels were determined by immunocytochemistry Scale bar = 10 µm. f Average HD-MB03 cell proliferation in the presence or absence of VEGFC in the culture medium. n = 3 experiments; p < 0.001 (ANOVA, with multiple comparisons).
