Fig. 3: Effect of VEGFC on Daoy and HD-MB03 cell aggressiveness.
From: VEGFC negatively regulates the growth and aggressiveness of medulloblastoma cells
a, b Assessment of the VEGFC production by VEGFCKO and VEGFC++ cells. VEGFC levels were measured in the cell culture media by ELISA assay. Daoy: n = 6 experiments; HD-MB03: n = 9 experiments; p < 0.001 (ANOVA, with multiple comparisons). c, d Daoy VEGFCKO and HD-MB03 VEGFC++ cell proliferation. c Daoy VEGFCKO cell proliferation was measured over 8 days and compared to control cells. n = 3 experiments; ANOVA: p < 0.001. d HD-MB03 VEGFC++ cell proliferation was measured over 8 days and compared to wild-type cells. n = 3 experiments; **: p < 0.01. e Immunoblot showing the involvement of the ERK pathway in the VEGFC-dependent cell proliferation. f, g Directional migration of Daoy VEGFCKO cells: (f) representative experiments (scale bar = 500 μm) and (g) average results. n = 4 experiments; ***: p < 0.001. h, i Directional migration of HD-MB03 cells: (h) representative experiments (scale bar = 500 μm) and (i) average results. n = 4 experiments; ***: p < 0.001. j, k Pseudo-tube formation in Daoy VEGFCKO cells: Representative experiments, scale bar = 1000 μm (j) and average results (k). n = 4 experiments; p < 0.001. l, m Pseudo-tube formation in HD-MB03 VEGFC++ cells: Representative experiments; scale bar = 200 μm (l) and average results (m). n = 4 experiments; p < 0.001.
