Fig. 4: Effects of irradiation and VEGFC on Daoy and HD-MB03 cell aggressiveness.
From: VEGFC negatively regulates the growth and aggressiveness of medulloblastoma cells
a VEGFC mRNA levels in Daoy or HD-MB03 irradiation-resistant cells (n = 6; p < 0.001). b VEGFC secretion by Daoy or HD-MB03 irradiation resistant cells (n = 4–6; ***: p < 0.001 vs. Daoy; $$$: p < 0.001 vs. HD-MB03). c Proliferation of Daoy and HD-MB03 irradiation-resistant cells with respect to their own non-irradiated control cells. Cells were counted every day over 8 days. n = 3; ANOVA: p < 0.001. d Directional migration of Daoy irradiation-resistant cells: representative experiments and average results (n = 4; p < 0.001). e, f Pseudo-tube formation in Daoy or HD-MB03 irradiation-resistant cells: representative experiments and average results (n = 5). Daoy (e) and HD-MB03 (f) pseudo-tube formation was significantly altered by irradiation (**: p < 0.01; ***: p < 0.001, respectively). g qPCR expression of VEGFC receptors in Daoy irradiation-resistant cells. n = 6. h qPCR expression of VEGFC receptors in HD-MB03 irradiation-resistant cells. n = 6. i Relative expression of CD146 mRNA in Daoy and HD-MB03 cells (n = 6; p < 0.001). j Representative FACS measurement of CD146 membrane expression in Daoy control or irradiation resistant cells. k Representative FACS measurement of CD146 membrane expression in HD-MB03 control or irradiation resistant cells. l Average FACS measurement of CD146 membrane expression in Daoy and HD-MB03 cells (control vs irradiated). (n = 4; p < 0.001).
