Table 1 Values of the fluorescence quantum yield and excited-state lifetime of measured nonswitchable proteins in comparison with the literature values.
From: Absolute quantum yield measurements of fluorescent proteins using a plasmonic nanocavity
Protein | \({\Phi} _{meas}^{\,}\) | \({\Phi} _{lit}^{\,}\) | \(\tau _{meas}^{\,}\left( {ns} \right)\) | τlit(ns) |
---|---|---|---|---|
Citrine | 0.61 ± 0.01 | 3.3 | 3.636 | |
EGFP | 0.61 ± 0.01 | 2.6 | 2.4–2.729 | |
mCherry | 0.24 ± 0.02 | 1.6 | ||
Clover | 0.79 ± 0.01 | 0.7640 | 3.1 | 3.243 |
mEGFP | 0.59 ± 0.01 | 2.6 | 2.59 [45] | |
mKate2 | 0.42 ± 0.01 | 2.5 | ||
mKO2 | 0.65 ± 0.01 | 0.6247 | 3.5 | --- |
mNeonGreen | 0.76 ± 0.01 | 0.8043 | 3.0 | 3.143 |
mOrange2 | 0.56 ± 0.01 | 2.5 | 2.748 | |
mPapaya | 0.80 ± 0.01 | 0.8349 | 2.9 | --- |
mPlum | 0.12 ± 0.03 | 0.1046 | 1.0 | --- |
mRuby2 | 0.35 ± 0.02 | 2.4 | ||
mTurquoise2 | 0.89 ± 0.01 | 0.9350 | 3.8 | 4.050 |
TagRFP | 0.51 ± 0.01 | 0.4837 | 2.4 | 2.2–2.337 |
TagRFP-T | 0.45 ± 0.01 | 2.2 | 2.342 |