Table 2 Values of the fluorescence quantum yield and excited-state lifetime of measured photoswitchable proteins in comparison with the literature values.
From: Absolute quantum yield measurements of fluorescent proteins using a plasmonic nanocavity
Protein | \({\Phi} _{meas}^{100\% }\) | \({\Phi} _{meas}^{50\% }\) | \({\Phi} _{lit}^{\,}\) | \(\tau _{meas}^{100\% }\left( {ns} \right)\) | \(\tau _{meas}^{50\% }\left( {ns} \right)\) | \(\tau _{meas}^{10\% }\left( {ns} \right)\) | \(\tau _{lit}^{\,}\left( {ns} \right)\) |
|---|---|---|---|---|---|---|---|
Dreiklang | 0.47 ± 0.01 | 0.45 ± 0.01 | 0.4134 | 2.9 | 2.9 | 2.9 | --- |
Dronpa | 0.69 ± 0.01 | 0.71 ± 0.01 | 3.3 | 3.3 | 3.3 | --- | |
DronpaM159T | 0.22 ± 0.02 | 0.21 ± 0.01 | 0.2351 | 1.2 | 1.2 | 1.2 | 0.6–0.952 |
rsCherry | 0.22 ± 0.02 | 0.20 ± 0.01 | 1.2 | 1.2 | 1.2 | --- | |
rsCherryRev | 0.08 ± 0.03 | 0.10 ± 0.01 | 0.7 | 0.7 | 0.8 | --- | |
rsEGFP | 0.40 ± 0.01 | 0.40 ± 0.01 | 0.3654 | 1.8 | 1.8 | 1.8 | --- |
rsEGFP2 | 0.35 ± 0.02 | 0.34 ± 0.01 | 0.3055 | 2.0 | 2.0 | 2.0 | --- |
rsFastLime | 0.62 ± 0.01 | 0.59 ± 0.01 | 3.1 | 3.1 | 3.1 | --- |