Fig. 6: ICOSL binds both the N-term and the C-term thrombin-generated fragments of OPN. | Communications Biology

Fig. 6: ICOSL binds both the N-term and the C-term thrombin-generated fragments of OPN.

From: Osteopontin binds ICOSL promoting tumor metastasis

Fig. 6

a Western blot showing the OPN recombinant proteins (FL: full length, N and C-terminal fragments) probed with the anti-His-tag antibody (uncropped blot is shown Supplementary Fig. 6); b ELISA-based interaction assay. The graph shows the interaction of titrated amounts of soluble ICOSL-Fc with a fixed amount of OPN-FL, OPN N- or C-fragments coated on the plate. (black circle) shows OPN full length, (black square) OPN-N and (black triangle) OPN-C. Data are expressed as means ± standard error (n = 3 technical replicates).

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