Fig. 4: Leca2 mutation leads to changes in islet transcriptome. | Communications Biology

Fig. 4: Leca2 mutation leads to changes in islet transcriptome.

From: PAX6 mutation alters circadian rhythm and β cell function in mice without affecting glucose tolerance

Fig. 4

a Heat map displaying differentially expressed genes (DEGs) in isolated islets. Genes were filtered for fold-change >1.5× and FDR < 10%. n = 4. b A comparative analysis of DEGs in Leca2 mutants and known PAX6 targets. c Relative islet mRNA expression of Pax6, Ins2, Pcx, Ffar1, Mafa, and Ucn3. n = 4 (WT Ucn3 n = 3, Leca2 Pcx n = 3), ns non-significant, *p < 0.05, **p < 0.01, ***p < 0.001 Welch’s and Student’s t-test. d Representative immunofluorescence images for GLUT2 in pancreatic islets. n = 3. Scale bar, 50 µm. e Representative immunofluorescence images displaying insulin and glucagon-positive cells in islets and quantifications of α and β mass, and their ratio. n = 5, >80 islets per mouse were analyzed. Scale bar, 100 µm. *p < 0.05, **p < 0.01 Welch’s t-test and Student’s t-test. Ten- to twelve-week-old mice were used for all experiments. Error bars display ±s.d.

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