Fig. 4: TcAK1 and TcAK2 have different subcellular localization patterns. | Communications Biology

Fig. 4: TcAK1 and TcAK2 have different subcellular localization patterns.

From: Broad-complex transcription factor mediates opposing hormonal regulation of two phylogenetically distant arginine kinase genes in Tribolium castaneum

Fig. 4

a Immunofluorescence of TcAK1 and TcAK2. Midguts were attached to multi-well slides, fixed, permeabilized, and stained with DAPI to visualize the nuclei (column A), mitochondria-specific markers MitoTracker Red CMXRos (column B), and antibodies against TcAK1 or TcAK2 (column C). Merged and enlarged images are shown in column D and column E, respectively. b Western blots of fractionated cellular proteins, including cytoplasmic protein without mitochondria (Column A), mitochondrial protein (Column B), and nuclear protein (Column C). Western blot was conducted using antibody against TcAK1 or TcAK2 and subcellular-specific antibodies, including anti-α-Tubulin antibody (cytoplasmic marker), anti-COX IV antibody (mitochondrial marker), and anti-Histone H3 antibody (nuclear marker). Experiments were repeated at least three times with similar results.

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