Fig. 5: The regulation of TcAK1 and TcAK2 by 20E and JH III. | Communications Biology

Fig. 5: The regulation of TcAK1 and TcAK2 by 20E and JH III.

From: Broad-complex transcription factor mediates opposing hormonal regulation of two phylogenetically distant arginine kinase genes in Tribolium castaneum

Fig. 5

a The effects of knockdown of expression of Tcphantom, Tcshade, TcJHAMT, TcMet, and TcKr-h1 on the mRNA expression of TcAK1 and TcAK2. b The effects of treatments in vivo with 20E and JH III on the mRNA and protein expressions of TcAK1 and TcAK2 in larvae. c The effects of treatments in vitro with 20E and JH III on the promoter activity of TcAK1 and TcAK2 in Drosophila Schneider 2 (S2) cell line. These experiments were repeated at least three times with similar results. Data are expressed as mean ± SEM (n = 3 biologically independent replicates). Asterisks indicate differences statistically significant at **P < 0.01 and *P < 0.05 (Student’s t-test).

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