Fig. 6: TcBR-C mediates hormonal regulation of TcAK1 and TcAK2. | Communications Biology

Fig. 6: TcBR-C mediates hormonal regulation of TcAK1 and TcAK2.

From: Broad-complex transcription factor mediates opposing hormonal regulation of two phylogenetically distant arginine kinase genes in Tribolium castaneum

Fig. 6

a The effects of treatments in vivo with 20E and JH III on the mRNA expressions of TcBR-C in larvae. b The effects of knockdown of expression of TcBR-C on basal expression levels and hormornal regulation of TcAK1 and TcAK2. c The effects of overexpression of TcBR-C isoforms on TcAK1 and TcAK2 promoter activities in S2 cells. d The effects of overexpression of TcBR-C-z2 on the hormonal regulation of promoter activity of TcAK1 and TcAK2. e EMSAs of TcBR-C-z2 binding to the promoters of TcAK1 and TcAK2. Data are expressed as mean ± SEM (n = 3 biologically independent replicates). Histogram bars annotated with the same lowercase letters are not significantly different (one-way ANOVA, P < 0.05). Asterisks indicate differences statistically significant at **P < 0.01 and *P < 0.05 (Student’s t-test).

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