Fig. 3: Plant genome editing with long-range deletions by using CRISPR TiD.
From: Genome editing in plants using CRISPR type I-D nuclease
The detection of long-range deletion mutations in the SlIAA9 gene induced by the CRISPR TiD. Gene structure, gRNA positions, and the different primer sets to amplify the mutation are indicated. Numbers show the primer sets (1; 1st PCR, 2; nested PCR). The PCR amplified fragments separated on agarose gels are also shown in left panels. The results of large deletion mutations analyzed by the Sanger sequencing of the cloned DNA from the CRISPR TiD transgenic tomato calli are shown in right panels. The nucleotide positions from the PAM were indicated on the sequence. Arrows indicate the specific bands used for the cloning and sequencing. All results in the electrophoresis and sequence analysis are typical examples from the representative mutant plants generated by TiD.
