Fig. 3: The SchistoView graphical user interface (http://haddock9.sfsu.edu/schistoview/home).

Selected data at 2 h illustrate the hierarchical approach to visualization. a Heat map of Mahalanobis distances (d_M) for seven test drugs over an 11-point, 2.5-fold dilution series (2 nM–20 μM). The test drugs are K11777 (K7), PZQ (PZ), sunitinib (Su), staurosporine (St), imipramine (Im), simvastatin (Si), and metrifonate (Me). DMSO controls are in column 1 and the average d_M for each DMSO control is shown. A d_M of 1.61 is 3 SD from the DMSO control. Clicking on well B8 of the heat map (512 nM PZQ—the yellow square) populates b–g in the GUI. b Heat map of the effect sizes (ES) for each of 15 features (columns) plotted vs. static, rate, or frequency modes (rows); a “descriptor” is the combination of a given feature and mode. Clicking on a descriptor, e.g., frequency of change-in-length (the magenta square) populates c–f. c Calculated waveforms defined by the range of length (amplitude) and the frequency of length contraction. DMSO control worms (black line) are slower moving than those treated with 512 nM PZQ at 2 h (red line). d Histogram displaying the distribution of frequency-of-length contraction for DMSO controls (green) and PZQ-treated worms (orange). e Dosage bar graph depicting the ES for the frequency-of-length contraction after 2 h with 11 PZQ concentrations. f Time bar graph depicting the ES for the frequency-of-length contraction after PZQ (512 nM) treatment across the 3 days indicated. g First image from time-lapsed movie of well B8 highlighted in a; in the live SchistoView, the 30-frame movie is shown. h First image from time-lapsed movie of a DMSO-treated well; in the live SchistoView, the 30-frame movie is shown.