Fig. 1: Effect of Hesperadin on proliferation and viability of synchronized parasites.

Intraerythrocytic P. falciparum 3D7 control parasites (3% parasitaemia, 5% haematocrit) were treated with 500 nM hesperadin and sampled every 6 h after hesperadin treatment. a Growth curves of parasite cultures untreated (control, squares), treated with 500 nM Hesperadin (Hesperadin, triangles), and similarly treated but washed free of drug after 18 h (Hesperadin washout, circles). Grey symbols represent averaged counts from three independent biological experiments, each scored as three technical replicates. The average of three experiments is shown as black symbols, with a bar indicating the SE, except when the symbol is larger than the error bar. Data are available in Supplementary Data 1. b SYBR green staining of parasites from a. Note the stained material between nuclei in drug-treated parasites. c Fluorescence micrographs of viable (top row), methanol-treated (middle row), and Hesperadin-treated parasites (bottom row), stained with the intercalating dyes Hoechst 33258 and propidium iodide. d Quantification of propidium iodide fluorescence in the images shown in d. Significant differences were calculated using two-tailed equal variance Students t-test, ****P < 0.0001. Images were captured with a Zeiss LSM 880 Confocal Laser Scanning Microscope (LSM). Scale bars apply to all micrographs and correspond to 5 µm.