Fig. 2: High-throughput identification of haploinsufficient and dosage-sensitive genes. | Communications Biology

Fig. 2: High-throughput identification of haploinsufficient and dosage-sensitive genes.

From: An inducible CRISPR interference library for genetic interrogation of Saccharomyces cerevisiae biology

Fig. 2

a Binned scatter plots of gene depletion score between the replicates. Pearson Correlation R value is reported for each pair. b Binned scatter plots of gene depletion score, limited to haploinsufficient genes, between the replicates. Pearson Correlation R value is reported for each pair. c The Receiver Operating Characteristic Curve (ROC) for the detection of haploinsufficient genes and FDR, TPR, and FPR trends based on decision values. ROC curve shows that the depletion z score is a strong classifier for haploinsufficient genes. The individual replicates are shown in gray. Area under the curve is 0.90. Dashed line denotes the decision value for FDR < 10%. d Violin plots depicting the gene depletion score distribution for the haploinsufficient genes, essential genes, nonessential genes and synthetic scrambled genes, resulting from 200× random sampling of synthetic randomly shuffled gRNAs, in induced (SC-His+ATc) versus uninduced samples (SC-His-ATc).

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