Fig. 1: Identification of C-terminal sequence of MSLN.
From: Multiple proteases are involved in mesothelin shedding by cancer cells
a MSLN was immunoprecipitated from cell culture media using mouse monoclonal Ab MN. The areas labeled MSLN were cut out and analyzed by LC-MS. A431 cells do not express MSLN. KB for KB31, KLM for KLM1; RH for RH16; OV for OVCAR8; H9 for A431/H9, an MSLN stably transfected line. IgG H, heavy chain. b C-terminal sequences of MSLN from various cell lines. A431/H9 sequences are from published data6. Arrows indicate cut sites.
