Fig. 4: Epithelial cellular relationships in patient #4.

A UMAP visualization of epithelial cells from patient #4 with color-coded for the corresponding sub-cluster (left) and the average inferred CNVs signals (right; gray to black). B Dot plots of the expression level of NE, urothelial-like, basal and luminal lineage markers across the populations shown in (A) (Source data are provided as Supplementary Data 1). C Immunofluorescence (IF) co-staining for K18 (red) and SYP (green) in sections for patient #4. Scale bar represents 100 μm. D The PAGA graph and connectivity scores of the populations shown in (A). E Velocities of epithelial cells from patient #4 are visualized as streamlines in a UMAP-based embedding, in which color-coded for the corresponding populations shown in (A). F Representative confocal fluorescence microscopy of triple co-staining of SYP (green), K18 (gray), and K5 (red) in PC TMA sections. The SYP + NE cells have three subtypes: K18⁺K5⁻SYP⁺, K18⁻K5⁻SYP⁺, and K18⁺K5⁺SYP⁺. Scale bars represent 25 μm. G Pie chart of statistics for PC TMA co-staining results showing that the major part of prostate cancers contain NE cells with exclusive luminal phenotype (K18⁺SYP⁺,83/102).