Table 2 Data collection and refinement statistics.

From: An improved fluorescent tag and its nanobodies for membrane protein expression, stability assay, and purification

 

TGP-Sb44

Data collection

 

Space group

P212121

Cell dimensions

 

  a, b, c (Å)

51.61, 83.49, 184.56

  α, β, γ (°)

90, 90, 90

Wavelength (Å)

0.97930

Resolution (Å)

49.53–2.03 (20.9–2.03)a

Rmerge

0.127 (1.694)

Rpim

0.037 (0.504)

I/σI

13.9 (1.6)

Completeness (%)

99.3 (91.3)

Multiplicity

12.8 (11.9)

CC*b

0.999 (0.896)

Refinement

 

Resolution (Å)

45.04–2.03

No. reflections

51,724

Rwork/Rfree

0.1753/0.2155

No. atoms

5990

 Protein

5435

 Ligand/ion

86

 Water

469

No. residues

670

B-factors (Å2)

39.40

 Protein

39.07

 Ligand/ion

36.65

 Water

43.78

R.m.s deviations

 

 Bond lengths (Å)

0.007

 Bond angles (°)

0.90

Ramachandran

 

 Favored (%)

98.48

 Allowed (%)

1.37

 Outlier (%)

0.15

PDB ID

6LZ2

  1. aHighest resolution shell is shown in parenthesis.
  2. b\(CC \ast = \sqrt {\frac{{2CC_{1/2}}}{{1 + CC_{1/2}}}}\)
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