Fig. 4: The SOM neuromodulator peptide is synthetized in the somatic region and packed into synaptic vesicles. | Communications Biology

Fig. 4: The SOM neuromodulator peptide is synthetized in the somatic region and packed into synaptic vesicles.

From: Somatostatin expressing GABAergic interneurons in the medial entorhinal cortex preferentially inhibit layerIII-V pyramidal cells

Fig. 4

a STED image of SOM immunoreactivity in a soma in the MEC. Note the granular labeling of putative endoplasmatic reticulum surrounding the empty nucleus. Scale: 2 μm. b SOM (left) and VGAT immunoreactivity detected with STED microscopy within the same synaptic boutons in MEC. Scale: 1 μm. c STED images of a Cre-dependent mCherry/ChR2-expressing bouton in the MEC of a SOM-Cre animal (top, red). SOM immunoreactivity in the same bouton shows granular, putative synaptic vesicle localization (bottom, green). Scale: 0.5 μm. d Electron microscopic image of a spine in layerII of the MEC innervated by two different type of boutons. B1 is a putative excitatory bouton with similar-sized synaptic vesicles. B2 is a bouton of mCherry/ChR2 (DAB precipitate) expressing SOM+ interneuron. Note the larger-sized synaptic vesicles (arrows) occurring among the normal-sized vesicles. Scale: 200 nm.

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