Fig. 2: The DNA-binding activity of Ustilago maydis Trf2. | Communications Biology

Fig. 2: The DNA-binding activity of Ustilago maydis Trf2.

From: Structurally distinct telomere-binding proteins in Ustilago maydis execute non-overlapping functions in telomere replication, recombination, and protection

Fig. 2

a The structure of human TRF1–DNA complex is used to illustrate the locations of three insertions within the Myb domain of U. maydis Trf2. The positions where the insertions occur are shown in light green. Note that these are all positioned away from the DNA–protein interface. b EMSA analysis was performed using varying concentrations of Trf2∆N-FG3 and double-stranded telomere oligonucleotides. A representative series of assays is shown and the quantified results (average ± SEM, n = 3 independent experiments) plotted on the bottom. c The DNA-binding specificity of Trf2 was examined using the same competitor oligonucleotides as in Fig. 1c. A representative series of assays is shown and the calculated results (average ± SEM, n = 3 independent experiments) plotted on the bottom.

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