Fig. 3: The organization of cowpea centromere analyzed by indirect immunostaining and structured illumination microscopy (SIM) in root cells.
From: Unequal contribution of two paralogous CENH3 variants in cowpea centromere function
a Both CENH3.1 (green) and CENH3.2 (red) occupy distinct domains at centromeres in interphase nuclei. b Prometaphase chromosomes. Partially overlapping CENH3.1 and CENH3.2 immunosignals of further enlarged centromere regions of (a) and (b) and of the extended chromatin fiber (c) suggest that centromeric nucleosome cluster contain either CENH3 variant. d, e CENH3.1 (red) and CENH3.2 (red) colocalizes with CENPC (green) at the centromeres of prometaphase chromosomes. Further enlarged centromere regions shown below are indicated with white boxes (a, b, d, e).
