Table 1 Reagents.
PN | Supplier | Working concentration | |
|---|---|---|---|
Inhibitors | |||
Gallein | 3090/50 | R&D Systems | 10 µM |
Dynasore | 324410 | Sigma | 100 µM |
MTA | D5011 | Thermo Fisher | 200 µM |
Antibodies | |||
ms-FLAG-647 | A01811-100 | Genscript | 1:1000 (FC/IF) |
rb-MYC | A190-105A | Bethyl Laboratories | 1:5000 (WB) |
rb-CXCR4 (UMB2) | Ab124824 | Abcam | 1:2000 (WB), 1:1000 (FC/IF) |
Rb-phospho-ERK1/2 | 4370 S | Cell Signaling Technologies | 1:2000 (WB) |
ms-total-ERK1/2 | 4696 S | Cell Signaling Technologies | 1:1000 (WB) |
rb-phospho-AKT S473 | 4060 S | Cell Signaling Technologies | 1:2000 (WB) |
rb-total AKT | C67E7 | Cell Signaling Technologies | 1:1000 (WB) |
rb-GM130 | 12480 S | Cell Signaling Technologies | 1:1000 (IF) |
ms-GAPDH | sc-47724 | Santa Cruz Biotechnology | 1:1000 (WB) |
STREP-568 | S11226 | Thermo Fisher | 1:5000 (WB) |
ms-CXCR4-APC (12G5) | FAB170A | R&D Systems | 1:200–1:2000 (FC) |
rb-β-arrestin-1 | 30036 S | Cell Signaling Technologies | 1:1000 (WB) |
gt-anti-rb Dylight 800 | SA5-35571 | Thermo Fisher | 1:5000 (WB) |
gt-anti-ms Dylight 680 | 35518 | Thermo Fisher | 1:5000 (WB) |
gt-anti-rb-488-AlexaFluor-Plus | A32731 | Thermo Fisher | 1:1000 (FC/IF) |
Gt-anti-rb-555 | 84541 | Thermo Fisher | 1:1000 (IF) |
Gt-anti-rb-Alexafluor-Plus | A32733 | Thermo Fisher | 1:1000 (IF) |
Gt-anti-ms-488 | A10680 | Thermo Fisher | 1:1000 (IF) |
Gt-anti-ms-647 | A21235 | Thermo Fisher | 1:1000 (IF) |
Rb-anti-EEA1 | 3288 S | Cell Signaling Technologies | 1:200 (IF) |
Rb-anti-Rab7 | 9367 S | Cell Signaling Technologies | 1:100 (IF) |
Biologics | |||
CXCL12 | 350-NS-050 | R&D Systems | 12.5 nM |
Pronase | 10165921001 | Sigma | 0.1% Solution |
Lambda phosphatase | sc-200312A | Santa Cruz Biotechnology | Manual instructions |
Others | |||
Streptavidin agarose | 20361 | Thermo Fisher | |
DAPI | D9542 | Sigma | 1 µg/ml |
NewBlot PVDF Stripping buffer | 928–40032 | LiCor | Manual instructions |
PVDF 0.22 µm membranes | IB401001 | Thermo Fisher | NA |
NHS-Sulfo-LC-biotin | 21335 | Thermo Fisher | 1 mg/ml |
ITAQ Universal SYBR Green | 1725121 | BioRad | Manual instructions |
iScript cDNA Synthesis Kit | 1706891 | RioRad | Manual instructions |
Quick-RNA miniprep | R1054 | Zymo Research | Manual instructions |
