Fig. 2: Cartilage-like tissues derived from BMSCs after 21 days in vitro differentiation with TGF-β1 exposure for the number of days indicated. | Communications Biology

Fig. 2: Cartilage-like tissues derived from BMSCs after 21 days in vitro differentiation with TGF-β1 exposure for the number of days indicated.

From: A single day of TGF-β1 exposure activates chondrogenic and hypertrophic differentiation pathways in bone marrow-derived stromal cells

Fig. 2

a Gross photographs and brightfield microscopy images of whole BMSC-derived tissues (scale bars for microscopy images, 1 mm), and corresponding diameters. b Toluidine blue stain of glycosaminoglycan (GAG) matrix in macro- and micro-pellet tissue cross-sections are shown for four unique BMSC donors (scale bars, 500 μm). c GAG, DNA, and normalized GAG/DNA quantification for each BMSC donor. Asterisks are shown for values that are significantly lower than the corresponding 21 days of TGF-β1 exposure control. Box plots: n = 4 for each donor; *P < 0.05.

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