Fig. 2: MTPA increases intratumoral T-cell infiltration.

a After minilaparotomy, the orthotopic HCC (red arrow) was elaborated to the skin surface. b, c NIR fluorescence prior to (b) and following (c) MTPA showed focal ablation of the right side of the tumor as a loss of ICG fluorescence (green arrow). d H&E staining shows patchy areas of necrosis (pink). E, CD8+ infiltration (brown cells) is increased following MTPA compared with a control tumor on immunohistochemistry (f). Flow cytometry (g, h) confirms the significant increase in CD3+ CD8+ cell infiltration with MTPA.