Fig. 1: Radiation resistant cell populations from two prostate cell lines have lower radiation sensitivity than parental counterparts.
a Surviving fraction at 2 Gy, as determined by clonogenic assay, for fluorescently labelled parental (white circles) and radioresistant (RR, black circles) populations from PC3 and DU145 cell lines. Surviving fraction values were normalised to 0 Gy. Bar indicates the median; data points represent biological replicates (n = 6). **P < 0.01, ***P < 0.001, as determined by unpaired, one-tailed, t test. b Growth curves of parental and RR populations grown as monolayers without and with radiation. Bar indicates the mean; data points represent biological replicates (n = 6 for 0 Gy; n = 4 for 2 and 6 Gy). *Padj < 0.05, **Padj < 0.01, ***Padj < 0.001, as determined by a mixed-effects model with Sidak correction. c Percent cell viability (normalised to untreated wells) of parental and RR populations grown as monolayers in response to increasing concentrations of cisplatin (PC3, n = 4 experiments; DU145, n = 3 experiments). The line indicates the best fit, concentration-response model (normalised response with variable slope). ***P < 0.001 for PC3 and DU145, as determined by extra sum-of-squares F-test.
