Fig. 4: Specimen evaluation of spatial transcriptome quality with sRIN assay.

a HE image (scale bar: 1 mm) and b sRIN heat map of a malignant childhood brain tumor diagnosed as anaplastic medulloblastoma grade IV (n = 4, technical replicates), boxes enclose regions shown in close-up. c HE image (scale bar: 1 mm) and d ST analysis showing unique genes, of the same childhood brain tumor (n = 2, technical replicates). Close-up of e HE (scale bar: 200 µm) image and f sRIN heat map of the same childhood brain tumor where deep blue areas represent intact cell rich tumor and in light purple areas the tumor is necrotic. The sRIN heat map show areas of varied sRIN values in the tumor areas and no sRIN values in necrotic areas, boxes enclose regions shown in close-up. Close-up of g HE (scale bar: 40 µm) image and h sRIN heat map of the same childhood brain tumor show areas of similar HE staining pattern and varied sRIN values.