Fig. 2: Genetic relationships between representative specimens from ten different European populations, as determined by whole-genome sequencing (WGS).

A Distance tree. Scale bar: 500 counts, representing 500 SNV from the set of common SNVs for all samples. The oldest known and likely foundational aquarium lineage of marbled crayfish is included as a reference (red star). B Bar plots illustrating the number of unique and shared SNVs. 6–9 and 2–5 indicate the number of SNVs that are shared by 6 to 9 and 2–5 animals, respectively. C Genome-wide analysis of variant allele frequency (VAF). Lines represent the average VAF per window of 10,000 heterozygous positions for each of the ten reference specimens. Variant allele frequencies are highly similar for all specimens and do not provide any evidence for loss of heterozygosity. The red line indicates the reference genome.