Fig. 2: Anterograde and retrograde ChR2 expression in target and input structures of the entopallium.
From: AAV1 is the optimal viral vector for optogenetic experiments in pigeons (Columba livia)
a Schematic illustration of the injection area in the entopallium. b Anterograde ChR2 expression in fibers projecting to the NI/MVL following injections of AAV1-CAG-ChR2. c Little anterograde ChR2 expression following injections of AAV1-hSyn-ChR2. d Schematic illustration of anterograde labeling in fibers projecting to the NI/MVL. e Schematic illustration of anterograde labeling in fibers projecting to the striatum. f Anterograde ChR2 expression in fibers projecting to the striatum following injections of AAV1-CAG-ChR2. g Little anterograde ChR2 expression in the striatum following injections of AAV1-hSyn-ChR2. h Schematic illustration of ChR2 expression in the nucleus rotundus, which is the main input region of the entopallium. i Extensive retrograde ChR2 expression in the nucleus rotundus following injections of AAV1-CAG-ChR2 into the entopallium. j Scarce retrograde ChR2 expression in the nucleus rotundus following injections of AAV1-hSyn-ChR2. All scale bars represent 200 µm. Note that images f, g, i, j have been rotated 90° to the left. Abbreviations: Arco arcopallium, E entopallium, GP globus pallidus, LSt lateral striatum, MSt medial striatum, MVL ventrolateral mesopallium, NI nidopallium intermedium, nRT nucleus rotundus, TrO tractus opticus.
