Fig. 3: Therapeutic cancer vaccine followed by radiation induced more leukocytes and CD8⁺ effector T cell infiltration in the tumors.

Established TC-1 tumors were treated by radiation alone or by the combinations of radiation with vaccines before or after radiation. The tumors were harvested on 14 days after RT. a Experimental procedures. b The tumor weights (left) and the percentage of CD45⁺ cells (right) in the tumors determined by FACS. c The percentage of CD8⁺ cells in CD45⁺ cells in the tumors, and d the percentage of CD8⁺IFN-γ⁺ cells in CD45⁺ cells in the tumors analyzed by flow cytometry. Experiments were repeated three times and two-tailed t-test was used for comparisons of biological replicates. Data are presented as mean ± SD; n = 4 mice/group. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001.