Fig. 4: Establishment and validation of a multi-tissue DNA methylation clock for broiler chicken.
From: A chicken DNA methylation clock for the prediction of broiler health
a PCA analysis based on the DNA methylation patterns of 67,651 LMRs in the training set (N = 36 samples). b PCA based on LMR methylation patterns after filtering and normalization. c Example of a highly weighted clock LMR from the Igfbp3 gene body (#5 in Tab. S4), showing progressive age-related methylation. Coordinates refer to chromosome 2 of chicken genome assembly (version 5.0). Green lines indicate methylation levels from 0 to 1. d Enrichment of clock LMRs and clock CpGs in specific genomic features. P promoters, G gene bodies, I intergenic regions. e Validation of LMR-based and CpG-based age prediction in breast muscle tissue from 6 independent animals belonging to 2 age groups (14 days and 28 days). Error bars indicate RMSEs.
