Fig. 5: Intestinal inflammation is associated with age acceleration in broiler chickens.
From: A chicken DNA methylation clock for the prediction of broiler health
a Schematic outline of the inflammation trial. After hatching (day 0), animals were periodically injected with CpG (or control GpC) oligonucleotide, as shown (orange tringles). At several timepoints (black circles), animals were euthanized and tissue was collected for analysis. b Inflammatory kinase activation in jejunum samples with experimentally induced inflammation. The number of observed differentially phosphorylated proteins in the top 10 immune related GO terms were counted for CpG injected broiler chickens at each day. At day 35 post-hatch, a substantial reduction in protein phosphorylation changes related to immunity was observed. c Age acceleration in jejunum samples with experimentally induced inflammation. The specific age acceleration in the d13-16 age group matches the immunological phenotype. Asterisks indicate statistically significant differences in relation to day 35 (P < 0.05, t-test).
