Fig. 2: Differentiation potential of prostate CSCs.
From: Chemosensitization of prostate cancer stem cells in mice by angiogenin and plexin-B2 inhibitors
a Expression levels of basal, luminal, and neuroendocrine markers in CSCs and parent cells measured by qRT-PCR (n = 3). Values in the CSCs were normalized to the respective parent cells. b Morphology of PC3 CSCs cultured in sphere medium in non-adherent petri dish (top) and in DMEM plus 10% FBS in cell culture dish (bottom) for 2 weeks. Scale bar, 500 μm. c mRNA levels of basal, luminal, and neuroendocrine markers in CSCs cultured in regular cell culture medium (DMEM + 10% FBS) for 3 and 8 days (n = 3). Heatmaps represent the relative mRNA level of each marker normalized to the value at day 1. d mRNA levels of basal, luminal, and neuroendocrine markers in CSCs and CSC-derived tumors (left), and in PC3 cells and PC3 cell-derived tumors (right) (n = 3). Values in the tumor tissues were normalized to the originating cells. IHC of CK18 (e) and SYP (f) in tumors derived from PC3 cells or CSCs. Quantitation of signal intensity was obtained by Image J (n = 3). Scale bar: left panels, 200 μm; right panels, 50 μm. ns not significant.
