Fig. 3: Knockdown of ANG or PLXNB2 decreases stemness of prostate CSCs.
From: Chemosensitization of prostate cancer stem cells in mice by angiogenin and plexin-B2 inhibitors
a mRNA levels of cancer stemness-related genes in prostate CSCs and parent cells (n = 5). Values in the CSCs were normalized to the respective parent cells. mRNA and protein levels of ANG (b) and PLXNB2 (c) in knockdown CSCs. mRNA levels were determined by qRT-PCR and normalized to control shRNA transfectants (n = 3). Protein levels were determined by immunoblotting. d mRNA level of cancer stemness-related genes in ANG and PLXNB2 knockdown CSCs (n = 3). Values were normalized to the control shRNA transfectants. Cell cycle status of ANG (e) and PLXNB2 (f) knockdown CSCs, analyzed by flow cytometry after Ki-67 and 7-AAD staining (n = 2). Sphere formation of ANG (g) and PLXNB2 (h) knockdown CSCs (n = 3). i CSC exhaustion during serial passaging in vivo. Cells were passaged in NSG mice (n = 5–8) for three times. In each passage, 100,000 cells were inoculated per mouse. Tumors were excised and weighed 4 weeks post inoculation in each passage. Exhaustion rate was calculated as the ratio of tumor weight from first passage to third passage. *p < 0.05; **p < 0.01; ***p < 0.001.
