Fig. 7: ANG mediates production of 3′-end fragment of 5S rRNA in prostate CSCs.
From: Chemosensitization of prostate cancer stem cells in mice by angiogenin and plexin-B2 inhibitors
a Small RNA in CSCs and parent cells analyzed by polyacrylamide gel electrophoresis and SYBR Gold staining (n = 3). Band intensity was determined by Image J and presented as relative values normalized to respective parent cells. b Effect of ANG on small RNA production in CSCs and PC3 cells (n = 3). Band intensity was determined by Image J and presented as relative values normalized to PBS-treated CSCs. c Northern blot analysis of tRNA-Gly-GCC (n = 2). d Northern blot analysis of small RNA from prostate CSCs and respective parent cells using the 3′-end fragment of 5S rRNA as the probe (n = 3). Band intensity was determined by Image J and presented as relative values normalized to respective parent cells. e Level of the 3′-end fragment of 5S rRNA in PLXNB2 and ANG knockdown CSCs analyzed by northern blotting, quantified by Image J, and normalized to control shRNA transfectants (n = 3). *p < 0.05; **p < 0.01; ***p < 0.001; ns not significant.
