Fig. 8: 3′-end fragment of 5S rRNA enhances stemness of prostate CSCs.
From: Chemosensitization of prostate cancer stem cells in mice by angiogenin and plexin-B2 inhibitors
a mRNA level of stemness-related genes in stable CSC transfectants of various small RNA (n = 3). Values were normalized to scramble RNA transfectants. b Sphere formation of stable CSC transfectants of various small RNA (n = 2). Scale bar: 500 μm. c Relative protein synthesis rate in stable transfectants of CSCs with various small RNA measured by OP-Puro incorporation followed by flow cytometry (n = 3). d mRNA level of stemness-related genes in stable transfectants of prostate CSCs of DU145 and LNCaP with scramble RNA control and the 3′-end fragment of 5S rRNA. e Xenograft growth of CSC transfectants of various small RNA. NSG mice were inoculated with 100,000 CSCs transfected with scramble RNA, intact 5S rRNA, the 5′- or 3′-end fragment of 5S rRNA, and treated with a single dose 30 mg/kg DTX (i.p.) on day 39. Bar graphs represent percent of inhibition of tumor growth in response to DTX treatment by comparing the tumor volume on day 45 to that on day 39 in each group. *p < 0.05; **p < 0.01; ***p < 0.001; ns not significant.
