Fig. 3: IFT20 deletion results in the disarrayed collagen fiber with the reduced strength in the cortical bone. | Communications Biology

Fig. 3: IFT20 deletion results in the disarrayed collagen fiber with the reduced strength in the cortical bone.

From: Primary cilia control cell alignment and patterning in bone development via ceramide-PKCζ-β-catenin signaling

Fig. 3

Micrographs representing SEM images of collagen fibrils obtained using Environmental Scanning Electron Microscope, XL30, FEI (Hillsboro, Oregon) with a beam of 5 kV. Longitudinal sections were visualized with magnification of ×50,000 (a–d). Longitudinal cryosection of tibia cortical bone from the tamoxifen treated IFT20f/f control mice (a) and IFT20f/f Col1cre-ERT mice (b) of 1-month-old were shown. Tibia from either Osx-cre controls (c) or IFT20f/f; Osx-cre mice (d). Femurs of 1-month-old were isolated from either IFT20f/f control mice (n = 3) or IFT20f/f Col1cre-ERT (n = 3) mice and the bone strength represented by maximum load (e) and bone stiffness (f) was measured using electromechanical testing machine (Instron 5542, Instron Inc., Norwood, MA) in the Penn Center for Musculoskeletal Disorders Biomechanics Core.

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